Metcn [FAST]

  • Set up tools:
  • Load the model, inspect S matrix, reactions, and gene–reaction associations.
  • Run basic analyses:
  • Visualize:

    • MET-CN is orchestrated by sensing mechanisms that monitor metabolite levels (e.g., 2-oxoglutarate as a C/N signal), energy status (AMP/ATP), and amino acid pools. Signaling hubs (TOR, AMPK, GCN2) integrate inputs to modulate anabolic/catabolic balance. Engineering strategies: modify 2-oxoglutarate sensors, optimize glutamine synthetase regulation, or rewire transporters to improve nitrogen use efficiency in crops. Challenges include compartmentalization and temporal dynamics.

    A functional MET CN includes: