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Downloaded over 30,000 times and cited in over 500 peer-reviewed publications. AltAnalyze has hundreds of active users and is being actively developed as an open-source project.

AltAnalyze and dependent applications recieve funding from NIH National Cancer Institute (R01CA226802 and R21CA300922).

 


Manuscripts to Cite (Open Access Links)

AltAnalyze
AltAnalyze3
OncoSplice
Splicing NeoAntigen Finder
RNA-SPRINT
MultiPath-PSI
ICGS2
cellHarmony
DoubletDecon

 

 
AltAnalyze - Comprehensive Transcriptome Analysis

AltAnalyze is an easy-to-use application for the end-to-end analysis of single-cell (ICGS/ cellHarmony) and bulk RNA-Seq data. For splicing sensitive platforms (single cell/bulk RNA-Seq or microarrays), AltAnalyze identities alternative splicing events,impacted protein isoforms, domain composition and microRNA targeting. AltAnalyze automates every step of gene expression and splicing analysis other data (FASTQ processing, RMA summarization, batch-effect removal, QC, statistics, annotation, clustering, network creation, lineage characterization, alternative exon visualization, gene-set enrichement and more). AltAnalyze3 contains special methods for bulk and single-cell long-read analysis. Easy to follow video tutorials can be found here. Updates can be found on our blog and examples in our interactive browsers.

AltAnalyze can be run through an inutitive graphical user interface or command-line and requires no advanced knowledge of bioinformatics programs or scripting. Alternative regulated splicing events can be visualized in the context of proteins, domains, microRNA binding sites and using SashimiPlots in this software. For program details and to get answers to common questions, check out our Manual, Sample Data, Wiki, FAQ, Tutorials or User Group.

 

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A powerful story grabs attention, but information creates action. Campaigns should pair personal narratives with clear educational resources:

Despite the power of survivor stories and awareness campaigns, there is a dark side. The non-profit industrial complex is hungry for content. Too often, organizations engage in "trauma porn"—the graphic, voyeuristic detailing of a survivor's lowest moment without providing resources or context.

To understand why survivor-led campaigns work, we must first look at the brain. Neuroscientific research has shown that when we are presented with dry statistics, only two small areas of the brain—the Broca’s area and Wernicke’s area (language processing)—light up. However, when we listen to a story, everything changes. The motor cortex, the sensory cortex, and even the frontal lobe engage. The listener doesn't just hear the survivor; they simulate the experience. A powerful story grabs attention, but information creates

This is known as "neural coupling." When a survivor shares their journey from trauma to triumph, the audience feels a fraction of that fear, pain, and relief. Consequently, empathy is not requested; it is chemically induced.

Consider the evolution of the HIV/AIDS awareness movement. In the 1980s, fear-based campaigns featuring grim reapers and statistics about mortality rates led to stigma and denial. It was only when survivors like Ryan White and activists in ACT UP shared their daily realities—the medications, the discrimination, the will to live—that the public shifted from fear to action. The story became the vaccine against apathy. However, when we listen to a story, everything changes

While not a traditional narrative, the Ice Bucket Challenge was powered by micro-stories. As videos flooded social media, each participant shared a link or a brief story about a specific person they knew battling Amyotrophic Lateral Sclerosis. The result was not just $115 million for the ALS Association, but the funding that led to the discovery of a new gene associated with the disease. The viral chain of shared personal motivation (however silly the ice water was) proved that peer-to-peer storytelling is a fundraising colossus.

In the landscape of modern advocacy, data points are quickly forgotten, but a single voice trembling with truth can echo for generations. We live in the age of information overload, where statistics about disease, violence, and crisis flash across screens only to be scrolled past a millisecond later. Yet, there is one variable that consistently breaks through the noise: the human narrative. the psychology behind why stories stick

The symbiotic relationship between survivor stories and awareness campaigns has become the most potent engine for social change in the 21st century. Whether the cause is cancer research, sexual assault prevention, mental health destigmatization, or human trafficking, it is the survivors who transform abstract numbers into urgent, unignorable realities.

This article explores the anatomy of this relationship, the psychology behind why stories stick, and how modern campaigns are ethically harnessing survivor voices to save lives.



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